Addgene - pCMV5B ActR-IB Plasmid Data

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Plasmid 14050: pCMV5B ActR-IB

Gene/insert name:
ActR-IB
Alt name:
Activin receptor, type I B
Insert size:
1500
Species:
H. sapiens (human)
GenBank ID:
U14722
Entrez Gene:
ACVR1B (ALK4, SKR2, ACTRIB, ACVRLK4)
Vector backbone:
pCMV5B
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
4700
Cloning site 5':
ClaI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
CMV Forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Joan Massague
Terms and Licenses:
MTA

Comments:

pCMV5B is the same as pCMV5 except that EcoRI and BglII sites are removed from the polylinker. See author's map for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Activation of signalling by the activin receptor complex. Attisano et al (Mol Cell Biol. 1996 Mar . 16(3):1066-73. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14050" in your Materials and Methods section.