pET16b-Rnl2(1-249)K227Q
(Plasmid #14072)

• Depositing Lab: Thomas Tuschl
• Publication: Rnl2 K227Q mutant (unpublished)

Backbone

• Vector backbone: pET16b
• Backbone manufacturer: Novagn
• Backbone size w/o insert (bp): 5711
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Rnl2(1-249)
• Species: Phage T4
• Insert Size (bp): 750
• Mutation: changed Lys227 to Glutamine Gly112 is actually Cys112 compared to NCBI
• GenBank ID: NP_049790
• Tag / Fusion Protein:
  • His (10x) (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Stewart Shuman, Memorial Sloan-Kettering Institute
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry