Plasmid 14106: pAdEasy Flag GCN5

• Gene/insert name: GCN5
• Species: H. sapiens (human)
• Entrez Gene: KAT2A (GCN5, GCN5L2, PCAF-b, hGCN5)
  
• Fusion protein or tag: flag
• Terminal: N terminal on insert
• Vector backbone: pAdEasy
  (Search Vector Database)
• Vector type: Mammalian Expression, Adenoviral
• Backbone size w/o insert: 25000
• Cloning site 5': KpnI
• Site destroyed during cloning: No
• Cloning site 3': XhoI
• Site destroyed during cloning: No
• 5' sequencing primer: n/a
• Bacterial resistance(s) Kanamycin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: High Copy
• Sequence: View sequences (1)
• Principal Investigator: Pere Puigserver
• Terms and Licenses: MTA

Comments: 

Flag-GCN5 was cloned into the pAdTrack shuttle vector and adenovirus constructs were created by recombination of the shuttle vector and pAdEasy vector by electroporation into BJ5183-AD-1 bacteria (Stratagene).

AdEasy is a registered trademark of the Johns Hopkins University.

Article: GCN5 acetyltransferase complex controls glucose metabolism through transcriptional repression of PGC-1alpha. Lerin et al (Cell Metab. 2006 Jun . 3(6):429-38. PubMed)