pRSET FlipSuc-90u delta1
(Plasmid #14475)

• Depositing Lab: Wolf Frommer
• Publication: Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-83.

Backbone

• Vector backbone: pRSET-B
• Backbone size w/o insert (bp): 2897
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FlipThuE W283A
• Alt name: ECFP-sucBP-EYFP
• Alt name: Sucrose binding protein
• Alt name: AtThuE
• Species: Agrobacterium tumefaciens
• Insert Size (bp): 2800
• Mutation: N192D, W283A, 13 amino acids deleted from linkers
• Promoter: T7
• Tags / Fusion Proteins:
  • ECFP (N terminal on insert)
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Supplemental Documents:
  • Annotated GenBank file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html

FLIPsuc-90u{Delta}1 was created by sub-cloning AtThuE into FLIPglu-600µ{Delta}5 with KpnI, which created a construct with 13 amino acids deleted from the linkers.