Addgene - pRSET FLIPmal-2u Plasmid Data

Plasmid 14479: pRSET FLIPmal-2u

Gene/insert name:
FLIPmal
Alt name:
FLIPmalE-5AA
Alt name:
MBP
Insert size:
2700
Species:
E. coli
Fusion protein or tag:
ECFP
Terminal:
N terminal on insert
Fusion protein or tag:
EYFP
Terminal:
C terminal on insert
Mutation:
5AA
Vector backbone:
pRSET
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
2900
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
N/A List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Wolf Frommer
Terms and Licenses:
MTA

Comments:

For more information, see
http://carnegiedpb.stanford.edu/research/fromme...

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Visualization of maltose uptake in living yeast cells by fluorescent nanosensors. Fehr et al (Proc Natl Acad Sci U S A. 2002 Jul 23. 99(15):9846-51. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14479" in your Materials and Methods section.