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JP1520 PIK3CA E545K HA
(Plasmid #14571)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 14571 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Jeff Engelman
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    PIK3CA E545K
  • Alt name
  • Alt name
    p110 alpha
  • Species
    B. taurus (bovine)
  • Mutation
  • Entrez Gene
  • Tag / Fusion Protein
    • HA tag (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

Hemagglutinin (HA)–tagged bovine PIK3CA cDNA was subcloned into the pDNR-Dual vector (Clontech, Mountain View, CA), and site-directed mutagenesis (Quick Change, Stratagene, La Jolla, CA) was used to generate the E545K mutation. The cDNA was shuttled into the JP1520 retroviral vector using the BD Creator System (Clontech).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JP1520 PIK3CA E545K HA was a gift from Joan Brugge (Addgene plasmid # 14571 ; ; RRID:Addgene_14571)
  • For your References section:

    Breast cancer-associated PIK3CA mutations are oncogenic in mammary epithelial cells. Isakoff SJ, Engelman JA, Irie HY, Luo J, Brachmann SM, Pearline RV, Cantley LC, Brugge JS. Cancer Res. 2005 Dec 1. 65(23):10992-1000. 10.1158/0008-5472.CAN-05-2612 PubMed 16322248