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Plasmid 12525: pBabe puro HA PIK3CA E545K
  • PIK3CA E545K

  • phosphoinositide 3-kinase

  • PI3K

  • p110 alpha

  • 3277

  • H. sapiens (human)

  • PIK3CA (CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha)

  • HA

  • N terminal on insert

  • E545K. Tumor-derived allele.

  • pBabe puro
    (Search Vector Database)

  • Available at Addgene (plasmid #1764)

  • Mammalian Expression, Retroviral

  • 5150

  • BamHI

  • No

  • SalI

  • No

  • pBABE 5' List of Sequencing Primers

  • pBABE-3

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Puromycin

  • View sequences (5)
  • Jean Zhao

  • UBMTA

Comments: 

Mutation generated using Strategene Quickchange site-directed mutagenesis kit. See article for primer information.

Please note that the original cDNA from HIP differed from the sequence posted on NCBI. This cDNA has an A->G alteration at the 424th nucleotide, which changes the amino acid from Ile -> Val. It could be a SNP. No functional changes have been observed so far in signaling assays.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The oncogenic properties of mutant p110alpha and p110beta phosphatidylinositol 3-kinases in human mammary epithelial cells. Zhao et al (Proc Natl Acad Sci U S A. 2005 Dec 20. 102(51):18443-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12525" in your Materials and Methods section.