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pBabe puro HA PIK3CA
(Plasmid #12522)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12522 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe puro
  • Backbone manufacturer
    Available at Addgene (plasmid #1764)
  • Backbone size w/o insert (bp) 5150
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PIK3CA
  • Alt name
    phosphoinositide 3-kinase
  • Alt name
    PI3K
  • Alt name
    p110 alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3277
  • Entrez Gene
    PIK3CA (a.k.a. CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE-3
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

To construct a hemagglutinin (HA)-tagged allele of p110 alpha, a cDNA clone of human PIK3CA (obtained from the FLEXGene Repository of the Harvard Institute of Proteomics [HIP]) was subcloned into pBabepuro by PCR with primers containing HA-epitope coding sequence with subsequent restriction and ligation.

Please note that the original cDNA from HIP differed from the sequence posted on NCBI. This cDNA has an A->G alteration at the 424th nucleotide, which changes the amino acid from Ile -> Val. It could be a SNP. No functional changes have been observed so far in signaling assays.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe puro HA PIK3CA was a gift from Jean Zhao (Addgene plasmid # 12522)
  • For your References section:

    The oncogenic properties of mutant p110alpha and p110beta phosphatidylinositol 3-kinases in human mammary epithelial cells. Zhao JJ, Liu Z, Wang L, Shin E, Loda MF, Roberts TM. Proc Natl Acad Sci U S A. 2005 Dec 20. 102(51):18443-8. 10.1073/pnas.0508988102 PubMed 16339315