pAc5.1B-EGFP-DmNot1_J
(Plasmid #146709)

• Purpose: Insect Expression of DmNot1
• Depositing Lab: Elisa Izaurralde
• Publication: Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94. doi: 10.1093/nar/gks1078. Epub 2012 Nov 21.

Backbone

• Vector backbone: Unknown
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: DmNot1
• Species: D. melanogaster (fly)
• Mutation: Three non silent mutations N605S, K759M, G1999S and 11 silent mutations compared to the sequence given by NCBI: NM_001103772.2.
• Entrez Gene: Not1 (a.k.a. Dmel_CG34407, CG1874, CG1884, CG34407, Dmel\CG34407, Dmel_CG1874, Dmel_CG1884, NOT, NOT1, anon-EST:Liang-1.29, anon-WO0118547.221, clone 1.29, not1)

Cloning Information

• Cloning method: Unknown

Resource Information

• Supplemental Documents:
  • pAc5.1B-EGFP-DmNot1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the Addgene verified sequence may be slightly different compared to the depositor's reference sequences. These differences do not affect plasmid function. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact before placing your order to request that our lab fully sequence the plasmid.