pAFW-Ago1
(Plasmid #50553)

• Purpose: Expression of FLAG tagged Ago1 in S2 cell
• Depositing Lab: Yukihide Tomari
• Publication: Kawamata et al Nat Struct Mol Biol. 2009 Sep;16(9):953-60. doi: 10.1038/nsmb.1630. Epub 2009 Aug 16.

Backbone

• Vector backbone: pAFW
• Backbone manufacturer: The Drosophila Gateway™ Vector Collection
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Argonaute1
• Species: D. melanogaster (fly)
• Entrez Gene: AGO1 (a.k.a. Dmel_CG6671, AGO-1, Ago, Ago-1, Ago1, Agp1, CG6671, Dm Ago1, DmAgo1, Dmel\CG6671, MRE20, ago1, ago1-1, anon-WO0257455.29, argonaute1, dAGO1, dAgo1, dmAgo1, l(2)04845, l(2)4845, l(2)k00208, l(2)k08121)
• Promoter: Actin5C
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: AC5

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene origin is from Phillip Zamore at Univ. of Massachusetts.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The closest reference sequence for the Ago1 insert in this plasmid appears to be NM_079010.4, Argonaute-1, isoform B.