HA GSK3 beta K85A pcDNA3
(Plasmid #14755)

• Depositing Lab: Jim Woodgett
• Publication: Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GSK3 beta K85A
• Alt name: GSK3b
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Mutation: K85A. Kinase dead mutant.
• Entrez Gene: GSK3B
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 32 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Nru I site is added to the K->A mutation