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Plasmid 14754: HA GSK3 beta S9A pcDNA3
  • Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

  • GSK3 beta S9A

  • GSK3b

  • 1300

  • H. sapiens (human)

  • GSK3B ()

  • HA

  • C terminal on insert

  • S9A. Constitutively active mutant.

  • pcDNA3
    (Search Vector Database)

  • Invitrogen

  • Mammalian Expression

  • 5500

  • CMV-F List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Neomycin

  • View sequences (3)
  • View map

  • Jim Woodgett

  • UBMTA

Comments: 

S2A in the sequence does not affect function, used to introduce restriction enzyme site

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation. Stambolic et al (Biochem J. 1994 Nov 1. 303 ( Pt 3)():701-4. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14754" in your Materials and Methods section.