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HA GSK3 beta S9A pcDNA3
(Plasmid #14754)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    GSK3 beta S9A
  • Alt name
    GSK3b
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Mutation
    S9A. Constitutively active mutant.
  • Entrez Gene
    GSK3B
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

S2A in the sequence does not affect function, used to introduce restriction enzyme site

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA GSK3 beta S9A pcDNA3 was a gift from Jim Woodgett (Addgene plasmid # 14754)
  • For your References section:

    Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation. Stambolic V, Woodgett JR. Biochem J. 1994 Nov 1. 303 ( Pt 3)():701-4. PubMed 7980435