Addgene - HA GSK3 beta S9A pcDNA3 Plasmid Data

Plasmid 14754: HA GSK3 beta S9A pcDNA3

Gene/insert name:
GSK3 beta S9A
Alt name:
GSK3b
Insert size:
1300
Species:
H. sapiens (human)
Entrez Gene:
GSK3B ()
Fusion protein or tag:
HA
Terminal:
C terminal on insert
Mutation:
S9A. Constitutively active mutant.
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Jim Woodgett
Terms and Licenses:
MTA

Comments:

S2A in the sequence does not affect function, used to introduce restriction enzyme site

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation. Stambolic et al (Biochem J. 1994 Nov 1. 303 ( Pt 3)():701-4. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14754" in your Materials and Methods section.