pSicoR human DGCR8-2
(Plasmid #14770)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pSicoR PGK puro
  • Backbone size w/o insert (bp) 7700
  • Vector type
    Mammalian Expression, Lentiviral, RNAi, Cre/Lox
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    DGCR8 shRNA
  • Alt name
    DGCR8
  • Species
    H. sapiens (human)
  • Entrez Gene
    DGCR8 (a.k.a. LP4941, C22orf12, DGCRK6, Gy1, pasha)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

shRNA sequence: TGAAGCTCAT TACTTTATCA TTCAAGAGAT GATAAAGTAA TGAGCTTCTT TTTTC

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSicoR human DGCR8-2 was a gift from Tyler Jacks (Addgene plasmid # 14770)
  • For your References section:

    Impaired microRNA processing enhances cellular transformation and tumorigenesis. Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T. Nat Genet. 2007 Apr 1. ():. 10.1038/ng2003 PubMed 17401365