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Lox-stop-Lox p53 R172H
(Plasmid #14854)


Item Catalog # Description Quantity Price (USD)
Plasmid 14854 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    p53 R172H
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    Structural mutant R172H. (Murine p53 codon 172 corresponds to human codon 175).
  • Entrez Gene
    Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)
  • Tag / Fusion Protein
    • Lox-STOP-lox (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Conditional mutant alleles of p53 were generated by introducing the LoxP-flanked transcriptional STOP cassette into intron 1 of the p53 gene. Site-directed mutagenesis was used to introduce an arg→his mutation at codon 172.

Note: This is a large plasmid that can undergo recombination. Bacterial colonies will grow slowly. If there are different colony sizes on a plate, the smaller ones are more likely to contain the correct plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lox-stop-Lox p53 R172H was a gift from Tyler Jacks (Addgene plasmid # 14854 ; ; RRID:Addgene_14854)
  • For your References section:

    Mutant p53 gain of function in two mouse models of Li-Fraumeni syndrome. Olive KP, Tuveson DA, Ruhe ZC, Yin B, Willis NA, Bronson RT, Crowley D, Jacks T. Cell. 2004 Dec 17. 119(6):847-60. 10.1016/j.cell.2004.11.004 PubMed 15607980