Addgene - Lox-stop-Lox p53 R172H Plasmid Data

Plasmid 14854: Lox-stop-Lox p53 R172H

Gene/insert name:
p53 R172H
Insert size:
17000
Species:
M. musculus (mouse)
Entrez Gene:
Trp53 (RP23-56I20.1, Tp53, bbl, bfy, bhy, p44, p53)
Fusion protein or tag:
Lox-STOP-lox
Terminal:
N terminal on insert
Mutation:
Structural mutant R172H. (Murine p53 codon 172 corresponds to human codon 175).
Vector backbone:
pBluescript
(Search Vector Database)
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert (bp):
3000
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

Conditional mutant alleles of p53 were generated by introducing the LoxP-flanked transcriptional STOP cassette into intron 1 of the p53 gene. Site-directed mutagenesis was used to introduce an arg→his mutation at codon 172.

Note: This is a large plasmid that can undergo recombination. Bacterial colonies will grow slowly. If there are different colony sizes on a plate, the smaller ones are more likely to contain the correct plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mutant p53 gain of function in two mouse models of Li-Fraumeni syndrome. Olive et al (Cell. 2004 Dec 17. 119(6):847-60. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14854" in your Materials and Methods section.