DAGR
(Plasmid #14865)

• Depositing Lab: Alexandra Newton
• Publication: Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DAG reporter
• Alt name: CFP-C1A-C1B-YFP
• Alt name: PKC beta II
• Species: R. norvegicus (rat)
• Entrez Gene: Prkcb (a.k.a. Pkcb, Prkcb1)
• Tags / Fusion Proteins:
  • CFP (N terminal on insert)
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CFP and YFP fused to the C1A and C1B domains of PKCbetaII. DAGR was created by PCR of the C1A and C1B domains of rat PKCßII (aa 37–152) from KpnI to XhoI and substitution for the PH domain in CYPHR.