Addgene - MyrPalm-CFP Plasmid Data

Plasmid 14867: MyrPalm-CFP

Gene/insert name:
CFP
Fusion protein or tag:
Myr/Palm
Terminal:
N terminal on insert
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Alexandra Newton
Terms and Licenses:
MTA

Comments:

MyrPalm-CFP was made by PCR with the same 5' primer as used for MyrPalm-CKAR and a 3' primer to the COOH terminus of CFP with an Xba site.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. Violin et al (J Cell Biol. 2003 Jun 9. 161(5):899-909. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14867" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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