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pEBFP2-Nuc
(Plasmid #14893)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14893 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEYFP-Nuc
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pEBFP2-Nuc
  • Alt name
    EBFP2 blue fluorescent protein with NLS
  • Species
    evolved fluorescent protein
  • Insert Size (bp)
    720
  • Mutation
    Replace EYFP of the vector pEYFP-Nuc from Clontech with EBFP2.
  • GenBank ID
    EF517318
  • Tag / Fusion Protein
    • NLS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F (vector primer) EGFP-N (1st MCS)
  • 3′ sequencing primer SV40pA-R (vector primer) EGFP-C (2nd MCS)
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBFP2-Nuc was a gift from Robert Campbell (Addgene plasmid # 14893)
  • For your References section:

    Exploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins. Ai HW, Shaner NC, Cheng Z, Tsien RY, Campbell RE. Biochemistry. 2007 Apr 20. ():. 10.1021/bi700199g PubMed 17444659