Addgene - pEBFP2-Nuc Plasmid Data

Plasmid 14893: pEBFP2-Nuc

Gene/insert name:
pEBFP2-Nuc
Alt name:
EBFP2 blue fluorescent protein with NLS
Insert size:
720
Species:
evolved fluorescent protein
GenBank ID:
EF517318
Fusion protein or tag:
NLS
Terminal:
C terminal on backbone
Mutation:
Replace EYFP of the vector pEYFP-Nuc from Clontech with EBFP2.
Vector backbone:
pEYFP-Nuc
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4000
Cloning site 5':
NheI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F (vector primer) EGFP-N (1st MCS) List of Sequencing Primers
3' sequencing primer:
SV40pA-R (vector primer) EGFP-C (2nd MCS)
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (4)
Principal Investigator:
Robert Campbell
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Exploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins. Ai et al (Biochemistry. 2007 Apr 20. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14893" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

14894: pmKalama1-Nuc

14891: pBad-EBFP2

18879: pmAmetrine-DEVD-tdTomato

15214: Cerulean

14892: pBad-mKalama1