Addgene - pmAmetrine-DEVD-tdTomato Plasmid Data

Plasmid 18879: pmAmetrine-DEVD-tdTomato

Gene/insert name:
mAmetrine-DEVD-tdTomato
Alt name:
caspase-3 sensor
Insert size:
2100
Species:
H. sapiens (human)
Species:
lab constructed
Mutation:
mAmetrine-tdTomato linker sequence: ...ITLGGTGSGSGDEVDGMVSKGEEVIK...; Backbone NLS tag has been removed when digested with BamH1
Vector backbone:
pEYFP-Nuc
(Search Vector Database)
Backbone manufacturer:
Clonetech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4000
Cloning site 5':
Nhe1
Site destroyed during cloning:
No
Cloning site 3':
BamH1
Site destroyed during cloning:
No
5' sequencing primer:
CGTCGCCGTCCAGCTCGACCAG List of Sequencing Primers
3' sequencing primer:
CATGGTCCTGCTGGAGTTCGTG
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Principal Investigator:
Robert Campbell
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Ai et al (Nat Methods. 2008 May . 5(5):401-3. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18879" in your Materials and Methods section.