Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pAJ13
(Plasmid #14915)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14915 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    multiple cloning site 3
  • Alt name
    MCS3
  • Species
    S. stercoralis (nematode)
  • Insert Size (bp)
    20

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site AvrII (not destroyed)
  • 5′ sequencing primer M13 forward
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

derived from pAJ02 with Mulitple cloning site 3 (MCS3).
See Fire Lab C. elegans Vector Kit 1995 Documetation.

PAPER:Int J Parasitol. 2006 May 31;36(6):671-9. Epub 2006 Feb 7 Successful transgenesis of the parasitic nematode Strongyloides stercoralis requires endogenous non-coding control elements.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAJ13 was a gift from James Lok (Addgene plasmid # 14915 ; http://n2t.net/addgene:14915 ; RRID:Addgene_14915)