pAJ50
(Plasmid #14918)

• Depositing Lab: James Lok
• Publication: Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Backbone

• Vector backbone: pAJ08
• Backbone size w/o insert (bp): 4515
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: enhanced monomeric red fluorescent protein
• Alt name: mRFPmars
• Alt name: synthetic RFP
• Species: Dictyostelium discoideum
• Insert Size (bp): 682
• GenBank ID: AY679163

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: AvrII (not destroyed)
• 5′ sequencing primer: M13

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Provided in by Dr. Jakob Franke at the Dicty Stock Center, Columbia University (http://dictybase.org/stockcenter/stockcenter.html). Paper: FEBS Letters 577 (2004) 227–232. A brilliant monomeric red fluorescent protein to visualize cytoskeleton dynamics in Dictyostelium.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PAPER:Int J Parasitol. 2006 May 31;36(6):671-9. Epub 2006 Feb 7 Successful transgenesis of the parasitic nematode Strongyloides stercoralis requires endogenous non-coding control elements.