Addgene - pBABE-puro-DN-hTERT Plasmid Data

Plasmid 1775: pBABE-puro-DN-hTERT

Gene/insert name:
hTERT
Alt name:
telomerase reverse transcriptase
Insert size:
3500
Species:
H. sapiens (human)
Entrez Gene:
TERT (CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
Mutation:
Dominant negative, 2 base pair mutation, catalytically dead
Vector backbone:
pBABE-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Inhibition of telomerase limits the growth of human cancer cells. Hahn et al (Nat Med 1999 Oct;5(10):1164-70. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1775" in your Materials and Methods section.