Addgene - pRK5 HA GST Rheb1 Plasmid Data

Plasmid 14951: pRK5 HA GST Rheb1

Gene/insert name:
Rheb1
Insert size:
580
Species:
H. sapiens (human)
Entrez Gene:
RHEBP1 (RHEB, RHEB1)
Fusion protein or tag:
HA-GST
Terminal:
N terminal on backbone
Vector backbone:
HA-GST-pRK5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5300
Cloning site 5':
SalI
Site destroyed during cloning:
Unknown
Cloning site 3':
NotI
Site destroyed during cloning:
Unknown
5' sequencing primer:
pGEX 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase. Sancak et al (Mol Cell. 2007 Mar 23. 25(6):903-15. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14951" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

14952: pRK5 HA GST rap2a

1861: myc-mTOR

14950: pRK5 Flag PRAS40

8513: HA Raptor

1859: myc-Raptor