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pGBW-m4137384
(Plasmid #149543)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 149543 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

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Backbone

  • Vector backbone
    AmpR gene, with start and stop codons
  • Backbone size w/o insert (bp) 860
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 S (surface glycoprotein)
  • Alt name
    Spike protein
  • Alt name
    S protein
  • Species
    H. sapiens (human); Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    6619
  • Mutation
    H. sapiens recode
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter CMV

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GinkgoBioworks
  • Terms and Licenses
  • Industry Terms

Depositor Comments

NCBI reference sequence: YP_009724390.1 . This plasmid contains an 18 or 19bp truncation in the CMV promoter and was synthesized for making pseudotyped VSV particles. It has been shown that truncations either help expression and /or capture by VSVdeltaG. See https://www.ncbi.nlm.nih.gov/pubmed/19057867 for original paper on VSV pseudotyping, and https://www.cell.com/cell/pdf/S0092-8674(20)30229-4.pdf on more recent efforts with SARS-CoV-2.

Please note: Addgene NGS finds that the AmpR resistance marker is in the opposite orientation in the plasmid relative to the depositor's sequence. As the plasmid was able to grow in media with Ampicillin, this is not expected to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBW-m4137384 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 149543 ; http://n2t.net/addgene:149543 ; RRID:Addgene_149543)