|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14973||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6300
Vector typeMammalian Expression, Retroviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameTGFbeta1 shRNA
SpeciesM. musculus (mouse)
Insert Size (bp)64
Entrez GeneTgfb1 (a.k.a. TGF-beta1, TGFbeta1, Tgfb, Tgfb-1)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer H1 (Common Sequencing Primers)
See author's map for more information. Target sequence: GGAGACGGAATACAGGGCTT.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRS-shTGFbeta1 was a gift from Joan Massague (Addgene plasmid # 14973 ; http://n2t.net/addgene:14973 ; RRID:Addgene_14973)
For your References section:TGF-beta directly targets cytotoxic T cell functions during tumor evasion of immune surveillance. Thomas DA, Massague J. Cancer Cell. 2005 Nov . 8(5):369-80. 10.1016/j.ccr.2005.10.012 PubMed 16286245
Map uploaded by the depositor.