Addgene - pMSCV TbetaRII ecto Plasmid Data

Plasmid 14974: pMSCV TbetaRII ecto

Gene/insert name:
TbetaRII ecto
Alt name:
TbetaR-II
Insert size:
530
Species:
H. sapiens (human)
Entrez Gene:
TGFBR2 (AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TGFR-2, TGFbeta-RII)
Fusion protein or tag:
6xHis
Terminal:
C terminal on insert
Mutation:
ecto domain
Vector backbone:
pMSCV Puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
6300
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
pLXSN5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Joan Massague
Terms and Licenses:
MTA

Comments:

Soluble TbetaRII. See author's map for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: TGF-beta directly targets cytotoxic T cell functions during tumor evasion of immune surveillance. Thomas et al (Cancer Cell. 2005 Nov . 8(5):369-80. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14974" in your Materials and Methods section.