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pCMV5 HA-TBRII (delta Cyt)
(Plasmid #14051)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14051 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TGF beta rec II (delta cyt)
  • Alt name
    TGF beta receptor, type II
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • Mutation
    Dominant negative mutant. A stop codon was introduced just downstream of the transmembrane domain, rendering it incapable of phosphorylating and activating the type I receptor.
  • GenBank ID
    NM_003242
  • Entrez Gene
    TGFBR2 (a.k.a. AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII)
  • Tag / Fusion Protein
    • HA (PYDVPDYA) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5 HA-TBRII (delta Cyt) was a gift from Joan Massague (Addgene plasmid # 14051 ; http://n2t.net/addgene:14051 ; RRID:Addgene_14051)
  • For your References section:

    Transforming growth factor beta signaling impairs Neu-induced mammary tumorigenesis while promoting pulmonary metastasis. Siegel PM, Shu W, Cardiff RD, Muller WJ, Massague J. Proc Natl Acad Sci U S A. 2003 Jul 8. 100(14):8430-5. 10.1073/pnas.0932636100 PubMed 12808151