Addgene - pCMV5 HA-TBRII (delta Cyt) Plasmid Data

Gene/insert name:
TGF beta rec II (delta cyt)
Alt name:
TGF beta receptor, type II
Insert size:
600
Species:
H. sapiens (human)
GenBank ID:
NM_003242
Entrez Gene:
TGFBR2 (AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TGFR-2, TGFbeta-RII)
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Mutation:
Dominant negative mutant. A stop codon was introduced just downstream of the transmembrane domain, rendering it incapable of phosphorylating and activating the type I receptor.
Vector backbone:
pCMV5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
4700
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CMV Forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Diagnostic digest (application/pdf)
Principal Investigator:
Joan Massague
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Transforming growth factor beta signaling impairs Neu-induced mammary tumorigenesis while promoting pulmonary metastasis. Siegel et al (Proc Natl Acad Sci U S A. 2003 Jul 8. 100(14):8430-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14051" in your Materials and Methods section.