|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14981||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
Growth in Bacteria
Gene/Insert namehuman frataxin promoter fragment
SpeciesH. sapiens (human)
Insert Size (bp)1941
Mutationincludes distal fragment of human frataxin promoter of appr. 2kb length
Entrez GeneFXN (a.k.a. CyaY, FA, FARR, FRDA, X25)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL3-basic-hFX-promotor(2kb, distal) was a gift from Michael Ristow (Addgene plasmid # 14981 ; http://n2t.net/addgene:14981 ; RRID:Addgene_14981)