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pGL3-basic-hFX-promotor(2,5kb)
(Plasmid #14980)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14980 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human frataxin promoter fragment
  • Alt name
    frataxin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2487
  • Mutation
    start codon for human frataxin gene has been mutated (ATG to AAG) to facilitate expression of reporter gene in vector; single XhoI site has been inserted after mutated start codon
  • Entrez Gene
    FXN (a.k.a. CyaY, FA, FARR, FRDA, X25)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer RVprimer3
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-basic-hFX-promotor(2,5kb) was a gift from Michael Ristow (Addgene plasmid # 14980 ; http://n2t.net/addgene:14980 ; RRID:Addgene_14980)