|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14979||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
Growth in Bacteria
Gene/Insert namehuman frataxin promoter fragment
SpeciesH. sapiens (human)
Insert Size (bp)1353
Mutationstart codon for human frataxin gene has been mutated (ATG to AAG) to facilitate expression of reporter gene in vector; single XhoI site has been inserted after mutated start codon
Entrez GeneFXN (a.k.a. CyaY, FA, FARR, FRDA, X25)
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL3-basic-hFX-promotor(1,3kb) was a gift from Michael Ristow (Addgene plasmid # 14979 ; http://n2t.net/addgene:14979 ; RRID:Addgene_14979)