miR-146a
(Plasmid #15092)

• Depositing Lab: David Baltimore
• Publication: Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 103(33):12481-6.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pre-miR-146a
• Alt name: microRNA miR-146a
• Alt name: cmv-146A
• Species: H. sapiens (human)
• Insert Size (bp): 330

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Genomic fragment corresponding to pre-miR-146a isoform was amplified from human genomic DNA and cloned into expression vector pcDNA3. When transfected into 293/IL-1R/TLR4/MD2 cells, this construct produces mature miRNA.