NFkBm2
(Plasmid #15090)

• Depositing Lab: David Baltimore
• Publication: Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 103(33):12481-6.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	15090	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pGL3-basic
  (Search Vector Database)
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: miR-146a promoter
• Alt name: microRNA miR-146a
• Species: H. sapiens (human)
• Insert Size (bp): 547
• Mutation: 2 NFkB binding sites mutated

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Resource Information

• Terms and Licenses:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 3 References

Depositor Comments

Two NFkappaB binding sites are mutated: GGGATTTCCC to accATTTCCC; GGGACTTTCC to accACTTTCC.