-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 15090 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepGL3-basic
-
Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4800
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemiR-146a promoter
-
Alt namemicroRNA miR-146a
-
SpeciesH. sapiens (human)
-
Insert Size (bp)547
-
Mutation2 NFkB binding sites mutated
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Resource Information
-
Terms and Licenses
-
Industry Terms
- Not Available to Industry
-
Articles Citing this Plasmid
Depositor Comments
Two NFkappaB binding sites are mutated: GGGATTTCCC to accATTTCCC; GGGACTTTCC to accACTTTCC.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
NFkBm2 was a gift from David Baltimore (Addgene plasmid # 15090 ; http://n2t.net/addgene:15090 ; RRID:Addgene_15090) -
For your References section:
NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses. Taganov KD, Boldin MP, Chang KJ, Baltimore D. Proc Natl Acad Sci U S A. 2006 Aug 15. 103(33):12481-6. 10.1073/pnas.0605298103 PubMed 16885212