TRAF6mut-UTR
(Plasmid #15094)

• Depositing Lab: David Baltimore
• Publication: Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 103(33):12481-6.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	15094	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pMIR-Report
  (Search Vector Database)
• Backbone manufacturer: Ambion
• Backbone size w/o insert (bp): 6470
• Vector type: Mammalian Expression, Luciferase
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRAF6 3'UTR
• Alt name: TRAF6
• Species: H. sapiens (human)
• Insert Size (bp): 500
• Mutation: 4-bp substitutions in the miR-146 target sites
• Entrez Gene: TRAF6 (a.k.a. MGC:3310, RNF85)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: Luc-F
• 3′ sequencing primer: M13-F20

Resource Information

• Terms and Licenses:
  • UBMTA
  • Luciferase Limited Use Label License
• Article Citing this Plasmid:
  • 1 Reference

Depositor Comments

Mutated version of luciferase reporter construct containing ~500 bp of 3' UTR of TRAF6 with 4-bp substitutions in the miR-146 target sites.