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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 15094 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMIR-Report
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Backbone manufacturerAmbion
- Backbone size w/o insert (bp) 6470
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Vector typeMammalian Expression, Luciferase
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRAF6 3'UTR
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Alt nameTRAF6
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SpeciesH. sapiens (human)
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Insert Size (bp)500
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Mutation4-bp substitutions in the miR-146 target sites
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Entrez GeneTRAF6 (a.k.a. MGC:3310, RNF85)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site MluI (not destroyed)
- 5′ sequencing primer Luc-F
- 3′ sequencing primer M13-F20 (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Article Citing this Plasmid
Depositor Comments
Mutated version of luciferase reporter construct containing ~500 bp of 3' UTR of TRAF6 with 4-bp substitutions in the miR-146 target sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TRAF6mut-UTR was a gift from David Baltimore (Addgene plasmid # 15094 ; http://n2t.net/addgene:15094 ; RRID:Addgene_15094) -
For your References section:
NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses. Taganov KD, Boldin MP, Chang KJ, Baltimore D. Proc Natl Acad Sci U S A. 2006 Aug 15. 103(33):12481-6. 10.1073/pnas.0605298103 PubMed 16885212