Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

RCASBP-AP (A) (CC#37)
(Plasmid #15160)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15160 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    RCASBP (A)
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; Avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PLAP
  • Alt name
    placental alkaline phosphatase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Entrez Gene
    ALPP (a.k.a. ALP, PALP, PLAP, PLAP-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    RCASBP(A) provided by S. Hughes.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

In order to clone PLAP into RCASBP vectors, the Clal2 adaptor plasmid described by Hughes et al. was used to obtain ClaI ends on PLAP. PLAP was removed from pDAP by SalI digestion and inserted into Clal2 at the SalI site. PLAP was removed from Clal2 by digestion with ClaI and inserted into RCASBP(A) at the ClaI site. Orientation of PLAP was checked by polymerase chain reaction amplification with an oligoprimer from the 3' untranslated end of PLAP (5'-GCCACCACCTACAGCCCA GTGG-3').

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCASBP-AP (A) (CC#37) was a gift from Connie Cepko (Addgene plasmid # 15160 ; http://n2t.net/addgene:15160 ; RRID:Addgene_15160)
  • For your References section:

    Replication-competent retroviral vectors encoding alkaline phosphatase reveal spatial restriction of viral gene expression/transduction in the chick embryo. Fekete DM, Cepko CL. Mol Cell Biol. 1993 Apr . 13(4):2604-13. PubMed 8455633