RCASBP-AP (A) (CC#37)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15160||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneRCASBP (A)
- Backbone size w/o insert (bp) 11600
Vector typeRetroviral ; Avian expression
Growth in Bacteria
Alt nameplacental alkaline phosphatase
SpeciesH. sapiens (human)
Insert Size (bp)2000
Entrez GeneALPP (a.k.a. ALP, PALP, PLAP, PLAP-1)
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byRCASBP(A) provided by S. Hughes.
Terms and Licenses
- Not Available to Industry
In order to clone PLAP into RCASBP vectors, the Clal2 adaptor plasmid described by Hughes et al. was used to obtain ClaI ends on PLAP. PLAP was removed from pDAP by SalI digestion and inserted into Clal2 at the SalI site. PLAP was removed from Clal2 by digestion with ClaI and inserted into RCASBP(A) at the ClaI site. Orientation of PLAP was checked by polymerase chain reaction amplification with an oligoprimer from the 3' untranslated end of PLAP (5'-GCCACCACCTACAGCCCA GTGG-3').
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RCASBP-AP (A) (CC#37) was a gift from Connie Cepko (Addgene plasmid # 15160 ; http://n2t.net/addgene:15160 ; RRID:Addgene_15160)
For your References section:Replication-competent retroviral vectors encoding alkaline phosphatase reveal spatial restriction of viral gene expression/transduction in the chick embryo. Fekete DM, Cepko CL. Mol Cell Biol. 1993 Apr . 13(4):2604-13. PubMed 8455633
Map uploaded by the depositor.