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G-RCAS (A)
(Plasmid #15182)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15182 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    RCAS (A)
  • Backbone size w/o insert (bp) 12000
  • Vector type
    Retroviral, RNAi ; Avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Grow in ccdB Survival or DB3.1 cells
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Gateway cassette

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

The RCAS-RNAi viruses have been modified so that they are now compatible with the Gateway cloning system (Invitrogen). DNA encoding the hairpin is cloned into a modified U6 vector using standard cloning techniques. The construct is then moved into the retroviral vector using Gateway reagents, thus bypassing the difficulties of cloning small fragments into RCAS. As a result, each construct can be made with a minimal number of cloning steps. See Supplemental Information of the article for a detailed protocol.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    G-RCAS (A) was a gift from Connie Cepko (Addgene plasmid # 15182 ; http://n2t.net/addgene:15182 ; RRID:Addgene_15182)
  • For your References section:

    RCAS-RNAi: a loss-of-function method for the developing chick retina. Harpavat S, Cepko CL. BMC Dev Biol. 2006 . 6():2. 10.1186/1471-213X-6-2 PubMed 16426460