Plasmid 15173: pCS6/U6

• Gene/insert name: None
• Vector backbone: pCMV Sport 6 with U6 promoter
  (Search Vector Database)
• Vector type: Mammalian Expression, RNAi
• Backbone size (bp): 5000
• 5' sequencing primer: SP6
• 3' sequencing primer: T3
• Bacterial resistance(s) Ampicillin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: High Copy
• Sequence: View sequences (1)
• Map: View map
• Principal Investigator: Connie Cepko
• Terms and Licenses: MTA

Comments: 

The pCS6/U6 vector was made by ligating an M13 PCR product from the pBS/U6 vector (from the Shi lab, Harvard Medical School) to a Rsr II-Mlu I blunted pCMV-Sport6 vector (Invitrogen). This vector is compatible with Invitrogen's Gateway system.

See Author's Map for cloning information and the article for a protocol for cloning hairpins into this vector (in the article's supplementary material).

Article: RCAS-RNAi: a loss-of-function method for the developing chick retina. Harpavat et al (BMC Dev Biol. 2006 . 6():2. PubMed)