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pSH1/M-FGFR3-Fv-Fvls-E
(Plasmid #15287)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15287 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSH1
  • Backbone manufacturer
    GR Crabtree lab
  • Backbone size w/o insert (bp) 3500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FGFR3 kinase, FKBP12v36
  • Alt name
    cek-2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2000
  • Mutation
    FGFR3 cytoplasmic domain only FKBP12 F36V
  • Entrez Gene
    Fgfr3 (a.k.a. CD333, FR3, Fgfr-3, Flg-2, HBGFR, Mfr3, sam3)
  • Tags / Fusion Proteins
    • ha (C terminal on insert)
    • Myristoylation-targeting domain c-Src (14aa) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI, SacII (not destroyed)
  • 3′ cloning site EcoRI, BamHI (not destroyed)
  • 5′ sequencing primer gaggtgttacttctgctctaaaagc
  • 3′ sequencing primer cactgcattctagttgtggtttg
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSH1/M-FGFR3-Fv-Fvls-E was a gift from David Spencer (Addgene plasmid # 15287 ; http://n2t.net/addgene:15287 ; RRID:Addgene_15287)
  • For your References section:

    Conditional activation of fibroblast growth factor receptor (FGFR) 1, but not FGFR2, in prostate cancer cells leads to increased osteopontin induction, extracellular signal-regulated kinase activation, and in vivo proliferation. Freeman KW, Gangula RD, Welm BE, Ozen M, Foster BA, Rosen JM, Ittmann M, Greenberg NM, Spencer DM. Cancer Res. 2003 Oct 1. 63(19):6237-43. PubMed 14559809