pGL2 (basic) 3xARE Lux
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14934||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepGL2 Basic
- Backbone size w/o insert (bp) 5600
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Alt nameactivin responsive region
Insert Size (bp)400
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
The A3-Luc reporter construct was generated by subcloning the region comprising the three AREs and the core promoter from the previously described A3-CAT construct (Huang et al. 1995, EMBO J 14(23):5965-5973.) into the pGL2-Basic luciferase vector (Promega).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL2 (basic) 3xARE Lux was a gift from Joan Massague (Addgene plasmid # 14934 ; http://n2t.net/addgene:14934 ; RRID:Addgene_14934)
For your References section:A mechanism of repression of TGFbeta/ Smad signaling by oncogenic Ras. Kretzschmar M, Doody J, Timokhina I, Massague J. Genes Dev. 1999 Apr 1. 13(7):804-16. 10.1101/gad.13.7.804 PubMed 10197981
Map uploaded by the depositor.