CROP-sgRNA-MS2
(Plasmid #153457)

• Purpose: CROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffold
• Depositing Lab: Wolf Reik
• Publication: Alda-Catalinas et al Cell Syst. 2020 Jul 22;11(1):25-41.e9. doi: 10.1016/j.cels.2020.06.004. Epub 2020 Jul 6.

Backbone

• Vector backbone: CROP-seq
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: None
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: sgRNA empty backbone
• gRNA/shRNA sequence: Filer

Resource Information

• Supplemental Documents:
  • CROP-sgRNA-MS2.gbk
• A portion of this plasmid was derived from a plasmid made by: The CROP-sgRNA-MS2 lentiviral backbone was synthesized by VectorBuilder by adapting the CROP-seq vector (Datlinger et al. 2017) with the following modifications: 1) the sgRNA scaffold sequence contains two MS2 loops that allow recruitment of MS2-p65-HSF1 in SAM ESCs; and 2) a fluorescent mCherry marker was included downstream of the EF1α promoter and linked through T2A to a puromycin resistance cassette, allowing assessment of the multiplicity-of-infection (MOI) by FACS and antibiotic selection of the cells
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry