pRetroX-TRE3G_FLAG-CRTC1/MAML2_PGK-GpNLuc
(Plasmid #154265)

• Purpose: Dox/Tet-inducible expression of the CRTC1-MAML2 fusion oncogene in mammalian cells.
• Depositing Lab: Antonio Amelio
• Publication: Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi: 10.1016/j.celrep.2021.108768.

Backbone

• Vector backbone: pRetroX-TRE3G-MCS_PGK-GpNLuc
• Backbone manufacturer: custom (Amelio Lab)
• Backbone size w/o insert (bp): 7193
• Total vector size (bp): 10307
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FLAG-CRTC1/MAML2 fusion oncogene
• Alt name: C1/M2
• Species: H. sapiens (human)
• Insert Size (bp): 3114
• Promoter: TRE3G
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: TRE3G-gatcgcctggagcaattccac

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry