Addgene - pK-myc-Par6C Plasmid Data

Plasmid 15474: pK-myc-Par6C

Gene/insert name:
Partitioning-defective protein 6C
Alt name:
Par6C
Alt name:
PAR-6C
Insert size:
1037
Species:
H. sapiens (human)
GenBank ID:
AF252292
Entrez Gene:
PARD6A (PAR6, PAR6C, TAX40, PAR-6A, TIP-40, PAR6alpha)
Fusion protein or tag:
Myc
Terminal:
N terminal on backbone
Vector backbone:
pKmyc
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
BamH1
Site destroyed during cloning:
No
Cloning site 3':
EcoR1
Site destroyed during cloning:
No
5' sequencing primer:
CAGGTCCAACTGCACCTCGGTTC List of Sequencing Primers
3' sequencing primer:
TTTGTGATGCTATTGCTTTATTTG TA
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Ian Macara
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42. Joberty et al (Nat Cell Biol 2000 Aug;2(8):531-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15474" in your Materials and Methods section.