Addgene - pcDNA3 Ash2L full Plasmid Data

Plasmid 15548: pcDNA3 Ash2L full

Gene/insert name:
Ash2L
Insert size:
1800
Species:
H. sapiens (human)
GenBank ID:
BC015936
Entrez Gene:
ASH2L (ASH2, ASH2L1, ASH2L2, Bre2)
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5446
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Kai Ge
Terms and Licenses:
MTA

Comments:

Full-length ASH2L cDNA was amplified by reverse transcription-PCR from HeLaS cells using primers 5'-TAT AGG ATC CTG GTG ATG GCG GCG GCA GGA G-3' and 5'-CAG CGA CTC GAG TCA GGG TTC CCA TGG GGG AC-3'.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex. Cho et al (J Biol Chem. 2007 Jul 13. 282(28):20395-406. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15548" in your Materials and Methods section.