Addgene - pCS2-UTX-F Plasmid Data

Plasmid 17438: pCS2-UTX-F

Gene/insert name:
UTX
Alt name:
ubiquitously transcribed tetratricopeptide repeat, X chromosome
Insert size:
4200
Species:
H. sapiens (human)
GenBank ID:
NM_021140
Entrez Gene:
KDM6A (RP13-886N14.3, KABUK2, UTX, bA386N14.2)
Fusion protein or tag:
6xMyc
Terminal:
N terminal on backbone
Fusion protein or tag:
Flag
Terminal:
C terminal on insert
Vector backbone:
pCS2+ NLSMT
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4400
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
SP6 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Kai Ge
Terms and Licenses:
MTA

Comments:

Full length human UTX with a C-terminal FLAG was PCR-amplified from MGC clone BC093868 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector.

Please see "Notes" for more information on this plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification of JmjC domain-containing UTX and JMJD3 as histone H3 lysine 27 demethylases. Hong et al (Proc Natl Acad Sci U S A. 2007 Nov 20. 104(47):18439-44. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17438" in your Materials and Methods section.

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