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pCS2-UTX-F
(Plasmid #17438)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCS2+ NLSMT
  • Backbone size w/o insert (bp) 4400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    UTX
  • Alt name
    ubiquitously transcribed tetratricopeptide repeat, X chromosome
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4200
  • GenBank ID
    NM_021140
  • Entrez Gene
    KDM6A (a.k.a. RP13-886N14.3, KABUK2, UTX, bA386N14.2)
  • Tags / Fusion Proteins
    • 6xMyc (N terminal on backbone)
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Full length human UTX with a C-terminal FLAG was PCR-amplified from MGC clone BC093868 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector.

There are several nucleotide differences, some that result in changes to the amino acid sequence, these are not know to effect the function of the plasmid. Please review Addgene's sequencing results for details.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-UTX-F was a gift from Kai Ge (Addgene plasmid # 17438)
  • For your References section:

    Identification of JmjC domain-containing UTX and JMJD3 as histone H3 lysine 27 demethylases. Hong S, Cho YW, Yu LR, Yu H, Veenstra TD, Ge K. Proc Natl Acad Sci U S A. 2007 Nov 20. 104(47):18439-44. 10.1073/pnas.0707292104 PubMed 18003914