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Plasmid 17438: pCS2-UTX-F
  • UTX

  • ubiquitously transcribed tetratricopeptide repeat, X chromosome

  • 4200

  • H. sapiens (human)

  • NM_021140

  • KDM6A (RP13-886N14.3, KABUK2, UTX, bA386N14.2)

  • 6xMyc

  • N terminal on backbone

  • Flag

  • C terminal on insert

  • pCS2+ NLSMT
    (Search Vector Database)

  • Mammalian Expression

  • 4400

  • XhoI

  • No

  • XbaI

  • No

  • SP6 List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • Unknown

  • View sequences (2)
  • View map

  • Kai Ge

  • UBMTA

Comments: 

Full length human UTX with a C-terminal FLAG was PCR-amplified from MGC clone BC093868 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector.

There are several nucleotide differences, some that result in changes to the amino acid sequence, these are not know to effect the function of the plasmid. Please review Addgene's sequencing results for details.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Identification of JmjC domain-containing UTX and JMJD3 as histone H3 lysine 27 demethylases. Hong et al (Proc Natl Acad Sci U S A. 2007 Nov 20. 104(47):18439-44. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17438" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only