PurposeExpression of UTX in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17438||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepCS2+ NLSMT
- Backbone size w/o insert (bp) 4400
Vector typeMammalian Expression
Growth in Bacteria
Alt nameubiquitously transcribed tetratricopeptide repeat, X chromosome
SpeciesH. sapiens (human)
Insert Size (bp)4200
Entrez GeneKDM6A (a.k.a. KABUK2, UTX, bA386N14.2)
/ Fusion Proteins
- 6xMyc (N terminal on backbone)
- Flag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Full length human UTX with a C-terminal FLAG was PCR-amplified from MGC clone BC093868 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector.
UTX contains K220E, V503A, N606D and T726K compared to reference sequence (NM_021140.3).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2-UTX-F was a gift from Kai Ge (Addgene plasmid # 17438 ; http://n2t.net/addgene:17438 ; RRID:Addgene_17438)
For your References section:Identification of JmjC domain-containing UTX and JMJD3 as histone H3 lysine 27 demethylases. Hong S, Cho YW, Yu LR, Yu H, Veenstra TD, Ge K. Proc Natl Acad Sci U S A. 2007 Nov 20. 104(47):18439-44. 10.1073/pnas.0707292104 PubMed 18003914