Addgene - MSCV JMJD3 Plasmid Data

Plasmid 21212: MSCV JMJD3

Gene/insert name:
JMJD3
Species:
H. sapiens (human)
Entrez Gene:
KDM6B (JMJD3)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Vector backbone:
pMSCVpuro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
6300
5' sequencing primer:
pLXSN-5 List of Sequencing Primers
3' sequencing primer:
MSCV-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Supplemental document:
Depositor notes and sequence (application/pdf)
Principal Investigator:
Paul Khavari
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Control of differentiation in a self-renewing mammalian tissue by the histone demethylase JMJD3. Sen et al (Genes Dev. 2008 Jul 15. 22(14):1865-70. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21212" in your Materials and Methods section.