Addgene - pRetrosuper Fbw7 shRNA-1 Plasmid Data

Plasmid 15660: pRetrosuper Fbw7 shRNA-1

Gene/insert name:
Fbw7 shRNA
Alt name:
Fbw7
Insert size:
64
Species:
H. sapiens (human)
Entrez Gene:
FBXW7 (AGO, CDC4, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4)
Vector backbone:
pRetrosuper
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral, RNAi
Backbone size w/o insert (bp):
6400
Cloning site 5':
BglII?
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
H1 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Martin Eilers
Terms and Licenses:
MTA

Comments:

hairpin targets the sequence: ACAGGACAGTGTTTACAAA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The ubiquitin-specific protease USP28 is required for MYC stability. Popov et al (Nat Cell Biol. 2007 Jul . 9(7):765-74. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15660" in your Materials and Methods section.