pCMV-Myc CDC4 WT*
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16652||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3800
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1500
Mutation*Note that this insert is only WT relative to the mutant version (www.addgene.org/16653). This is not full length CDC4. See comments and paper for specifics.
Entrez GeneFBXW7 (a.k.a. AGO, CDC4, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4)
/ Fusion Protein
- myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer EBV rev (Common Sequencing Primers)
A 1.5kb fragment of human CDC4 cDNA (nucleotides 374-1874), which encompasses the entire ORF, was cloned into pCMV-Myc.
Addgene's quality control sequence suggests there are some unexpected mutations in the gene. The effect these mutations have on function is unknown, but this is the plasmid used in the Vogelstein article, so it should behave as reported.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-Myc CDC4 WT* was a gift from Bert Vogelstein (Addgene plasmid # 16652 ; http://n2t.net/addgene:16652 ; RRID:Addgene_16652)
For your References section:Inactivation of hCDC4 can cause chromosomal instability. Rajagopalan H, Jallepalli PV, Rago C, Velculescu VE, Kinzler KW, Vogelstein B, Lengauer C. Nature. 2004 Mar 4. 428(6978):77-81. 10.1038/nature02313 PubMed 14999283