|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15670||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 8081
Vector typebacterial allelic exchange vector
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThis vector was generated by our lab, using a backbone (pCM184) previously developed by the PI (BioTechniques, 33:1062-1067).
Terms and Licenses
Ampicillin, Chloramphenicol, Tetracycline
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCM433 was a gift from Christopher Marx (Addgene plasmid # 15670 ; http://n2t.net/addgene:15670 ; RRID:Addgene_15670)
For your References section:Development of a broad-host-range sacB-based vector for unmarked allelic exchange. Marx CJ. BMC Res Notes. 2008 Feb 26;1:1. doi: 10.1186/1756-0500-1-1. 10.1186/1756-0500-1-1 PubMed 18710539