601L-145-8X
(Plasmid #158572)

• Purpose: (Empty Backbone) Production of DNA fragment for nucleosome core particle preparation
• Depositing Lab: Curt Davey
• Publication: Chua et al Nucleic Acids Res. 2012 Jul;40(13):6338-52. doi: 10.1093/nar/gks261. Epub 2012 Mar 26.

Backbone

• Vector backbone: pUC57
• Backbone manufacturer: EZbiolab
• Backbone size (bp): 2710
• Modifications to backbone: Insertion at EcoRV site
• Vector type: Bacterial vector for DNA production

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13 fwd
• 3′ sequencing primer: M13 rev

Resource Information

• Supplemental Documents:
  • 601L-145-8X

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please cite the following article in any publications where this construct is utilized:
E.Y.D. Chua, D. Vasudevan, G.E. Davey, B. Wu & C.A. Davey. 2012. The Mechanics Behind DNA Sequence-Dependent Properties of the Nucleosome. Nucleic Acids Res. 40(13): 6338-6352.