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pcDNA3-HtrA2-FLAG
(Plasmid #15938)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15938 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HtrA2
  • Alt name
    Omi
  • Alt name
    PRSS25
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1750
  • GenBank ID
    NM_145074
  • Entrez Gene
    HTRA2 (a.k.a. MGCA8, OMI, PARK13, PRSS25)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-Forward
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

This construct was used in the experiments described in figure 5

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HtrA2-FLAG was a gift from L. Miguel Martins (Addgene plasmid # 15938 ; http://n2t.net/addgene:15938 ; RRID:Addgene_15938)
  • For your References section:

    The mitochondrial protease HtrA2 is regulated by Parkinson's disease-associated kinase PINK1. Plun-Favreau H, Klupsch K, Moisoi N, Gandhi S, Kjaer S, Frith D, Harvey K, Deas E, Harvey RJ, McDonald N, Wood NW, Martins LM, Downward J. Nat Cell Biol. 2007 Sep 30. ():. 10.1038/ncb1644 PubMed 17906618