Addgene - pcDNA3-HtrA2-FLAG Plasmid Data

Plasmid 15938: pcDNA3-HtrA2-FLAG

Gene/insert name:
HtrA2
Alt name:
Omi
Alt name:
PRSS25
Insert size:
1750
Species:
H. sapiens (human)
GenBank ID:
NM_145074
Entrez Gene:
HTRA2 (OMI, PARK13, PRSS25)
Fusion protein or tag:
FLAG
Terminal:
C terminal on insert
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5446
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-Forward List of Sequencing Primers
3' sequencing primer:
BGH Reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
L. Miguel Martins
Terms and Licenses:
MTA

Comments:

This construct was used in the experiments described in figure 5

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The mitochondrial protease HtrA2 is regulated by Parkinson's disease-associated kinase PINK1. Plun-Favreau et al (Nat Cell Biol. 2007 Sep 30. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15938" in your Materials and Methods section.