Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16012||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5600
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)3600
Entrez GeneTNFRSF10B (a.k.a. CD262, DR5, KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9)
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer EBVrev
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
A 3.6 Kb Pst1 fragment from the KILLER/DR5 P1 clone which includes 1.6 Kb upstream of the ATG through Intron 2 in KILLER/DR5 genomic DNA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL2-Full was a gift from Wafik El-Deiry (Addgene plasmid # 16012 ; http://n2t.net/addgene:16012 ; RRID:Addgene_16012)
For your References section:Wild-type p53 transactivates the KILLER/DR5 gene through an intronic sequence-specific DNA-binding site. Takimoto R, El-Deiry WS. Oncogene. 2000 Mar 30. 19(14):1735-43. 10.1038/sj.onc.1203489 PubMed 10777207