Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16016||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1460
Entrez GeneCFLAR (a.k.a. CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, cFLIP)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
A 1,460-bp sequence containing the promoter region of the FLIP gene was amplified using the BAC clone RP11-536118 (Children’s Hospital Oakland Research Institute, Oakland, Calif.) and inserted into the luciferase-expressing reporter plasmid pGL3-basic (Promega). The sequence corresponds to positions –1179 to +281 relative to the proposed transcriptional start site of CFLAR (FLIP) exon 1 (accession no. AB038965).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL3-FLIP1500 was a gift from Wafik El-Deiry (Addgene plasmid # 16016 ; http://n2t.net/addgene:16016 ; RRID:Addgene_16016)
For your References section:Direct repression of FLIP expression by c-myc is a major determinant of TRAIL sensitivity. Ricci MS, Jin Z, Dews M, Yu D, Thomas-Tikhonenko A, Dicker DT, El-Deiry WS. Mol Cell Biol. 2004 Oct . 24(19):8541-55. 10.1128/MCB.24.19.8541-8555.2004 PubMed 15367674